Whole genome microarray and proteomics analysis of cacao embryogenesis

Maximova, Siela

Date: Sunday, January 15, 2012
Time: 5:15 PM

Room: Royal Palm Salons 3-4

Siela Maximova , The Pennsylvania State University, University Park, PA

Niemenak Niemenak , University of Yaounde I, Yaounde, Cameroon

Xiangling Shen , The Pennsylvania State University, University Park, PA

Monica Santos-Mendoza , The Pennsylvania State University, University Park, PA

Zi Shi , The Pennsylvania State University, University Park, PA

Yufan Zhang , The Pennsylvania State University, University Park, PA

Sergio L Florez , The Pennsylvania State University, University Park, PA

Wayne Curtis , The Pennsylvania State University, University Park, PA

Mark Guiltinan , The Pennsylvania State University, University Park, PA

To explore the mechanism and genes involved in somatic and zygotic embryogenesis of Theobroma cacao, a whole genome microarray was used to measure gene expression of several stages of embryo development. In addition, 2D-gel electrophoresis and mass spectrometry were used to survey some of the major proteins that were differently expressed. Significant qualitative and quantitative changes were detected in gene and protein expression patterns across the developmental stages and between zygotic and somatic embryos. The gene expression profiles of key seed-specific and embryo development transcription factors, seed storage proteins, fatty acid biosynthesis, and flavonoid genes were evaluated and 107 proteins expressed in pod, zygotic and somatic embryo tissues were identified. The results from this research will facilitate the identification of regulatory networks that are important for programming zygotic and somatic embryos development and also identification of zygotic and somatic embryogenesis markers.

W130 Whole genome microarray and proteomics analysis of cacao embryogenesis