W202 SNP-based genotyping in lentil: linking sequence information with phenotypes

Date: Saturday, January 14, 2012
Time: 1:50 PM
Room: Pacific Salon 6-7 (2nd Floor)
Kirstin Bett , University of Saskatchewan, Saskatoon, SK, Canada
Andrew Sharpe , National Research Council, Saskatoon, SK, Canada
Clarice J. Coyne , USDA-ARS/Washington State University, Pullman, WA
Rebecca McGee , USDA-ARS, Pullman, WA
Larissa D. Ramsay , National Research Council, Saskatoon, SK, Canada
Wayne E. Clarke , Agriculture and Agri-Food Canada, Saskatoon, SK, Canada
Lacey-Anne Sanderson , University of Saskatchewan, Saskatoon, SK, Canada
Michael Fedoruk , University of Saskatchewan, Saskatoon, SK, Canada
Bert Vandenberg , University of Saskatchewan, Saskatoon, SK, Canada
Lentil (Lens culinaris) has been late to enter the world of high throughput molecular analysis due to a general lack of genomic resources.  Using a 454 sequencing-based approach, SNPs have been identified in genes across the lentil genome.  Several hundred have been turned into single SNP KASP assays (KBioscience) and a 1536-SNP Illumina Golden Gate assay has been developed.  A collection of over 300 diverse lines has been genotyped to assess genetic diversity and structure in lentil germplasm.  A gene-based map of the lentil genome has been developed that has been used to identify regions of conserved synteny with Medicago truncatula.  Phenotypic evaluation of both bi-parental and association mapping populations is underway and is leading to the identification of both markers and genes associated with traits of interest to the lentil breeding program.
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