A Duet of NGS Sample Preparation Techniques

Rapid, accurate DNA fragmentation is a critical step in Next-Generation Sequencing (NGS) and the ability to rapidly shear and measure the quality of this step is a workflow bottleneck in the library preparation process.  A cost and time effective solution to address both the shearing and subsequent quantity/quality assessment is the use of this duet of newly released technologies: Bulk Lateral Ultrasonic (BLU™) Energy for DNA shearing and a fluorescence-based capillary electrophoresis instrument for both accurate sizing and quantifying nucleic acids. Both technologies avoid the excessive use of expensive consumables, overcoming shortcomings of most current technologies, while providing considerably higher throughput and reduced labor to meet the needs of emerging demand for Next-Generation Sequencing.  The fundamental technologies are explored, matched with data that will show the unique capabilities of this powerful, elegant duet.