In plant breeding, the ability to manipulate
genetic (meiotic) recombination would be beneficial for
facilitating gene transfer from wild relatives of crop plants.
The DNA mismatch repair (MMR) system helps maintain
genetic integrity by correcting base mismatches that arise
via DNA synthesis or damage, and antagonizes recombination
between homeologous (divergent) DNA sequences.
Previous studies have established that the genomes of
cultivated tomato (Solanum lycopersicum) and the wild
relative S. lycopersicoides are substantially diverged
(homeologous) such that recombination between their chromosomes
is strongly reduced. Here, we report the effects on
homeologous recombination of suppressing endogenous
MMR genes in S. lycopersicum via co-suppression, RNAi-induced silencing
of SlMSH2, and SlMSH7 or overexpressing dominant negatives
of Arabidopsis MSH2 (AtMSH2-DN) in alien
substitution/introgression lines (SL-8, SL-10 and SL7-F) of S. lycopersicoides in tomato.