W487 Leveraging on the Elaeis Genome and Transcriptome for Enhancing Agronomic Traits

Date: Sunday, January 15, 2012
Time: 1:35 PM
Room: Pacific Salon 4-5 (2nd Floor)
Rajinder Singh , Malaysian Palm Oil Board, Selangor, Malaysia
Meilina Ong Abdullah , Malaysian Palm Oil Board, Selangor, Malaysia
Leslie Eng Ti Low , Malaysian Palm Oil Board, Selangor, Malaysia
Rajanaidu Nookiah , Malaysian Palm Oil Board, Selangor, Malaysia
Nathan Lakey , Orion Genomics LLC, St. Louis, MO
Steve Smith , Orion Genomics LLC, St. Louis, MO
Arief Budiman , Orion Genomics LLC, St. Louis, MO
Jared Ordway , Orion Genomics LLC, St. Louis, MO
Dave Kudrna , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Rod A. Wing , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Robert Martienssen , Cold Spring Harbor Lab, Cold Spring Harbor, NY
Ravigadevi Sambanthamurthi , Malaysian Palm Oil Board, Selangor, Malaysia
The emergence of next generation sequencing technologies has resulted in more affordable and rapid genome sequencing. Using 454/Roche technology, the Malaysian Palm Oil Board (MPOB) sequenced the genome of E. guineensis and E. oleifera palms. Additionally, from a 12x BAC library, a high information content physical map and BAC end sequences were produced.  Genome assembly was enhanced by the inclusion of BAC end sequences. BAC fingerprint contigs were merged with sequence scaffolds and with an oil palm genetic map, to provide an integrated genome-wide sequence map of oil palm.  The reference genomes were annotated with 454-based transcriptome sequences of more than 30 tissues. Obtaining high quality sequence assembly provides a valuable resource for further improvement of the oil palm using genomics-guided strategies. Genome tiling arrays, designed from the sequence, are being used by MPOB to explore the role of epigenetic control in somaclonal variation and abnormality observed in clonal palms.