P0759 The Development of an Herbicide Resistant Transgenic Garlic (Allium sativum L.)

Yul Kyun Ahn , Agricultural Reaearch Center for Climate Change, National Institute of Horticultural & Herbal Science, RDA, Jeju, South Korea
Me Hea Park , Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA, Suwon, South Korea
Moo Kyoung Yoon , Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA, Suwon, South Korea
Jeong Ho Kim , Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA, Suwon, South Korea
Myeong Cheoul Cho , Vegetable Research Div., National Institute of Horticultural & Herbal Science, RDA, Suwon, South Korea
Garlic breeding has been limited to clonal selection of wild varieties or spontaneous mutants because most of this species does not form fertile flowers. Allium species have proved to be recalcitrant to genetic transformation. We developed an efficient transformation system for garlic using Agrobacterium tumefacience in producing herbicide resistant GM garlic lines. The weed is big problem in garlic cultivation. And also there is no proper herbicide for garlic. The herbicide GM garlic will be reducing input cost. We obtained transgenic plants in 'Danyang’, ‘Samchec’ and ‘Namdo’ by transformation. We planted transgenic garlic bulb in the green house, and investigated plant growth and stable expression of herbicide resistant gene. With regard to plant growth, most of the transgenic lines showed normal growth as same as non-transgenic Garlic and well grew to maturity with normal bulb. Integration of the Bar gene was confirmed by PCR and Southern blot analysis for the genomic DNA of the transgenic garlic plants. RT –PCR confirmed gene expression of herbicide resistant gene in transgenic garlic. For bio-assay for the transgenic plants, sample transgenic plants were sprayed with 0.5% Barstar. All the transgenic plants survived. We obtained TV2 transgenic garlic in 'Danyang and ‘Samchec’ and TV0 transgenic garlic in 'Namdo’