W395 Assembling the Diversity: Variety-Specific Reconstruction and Characterization of Berries Transcriptome in Vitis vinifera cv. Corvina

Date: Sunday, January 15, 2012
Time: 1:50 PM
Room: Pacific Salon 6-7 (2nd Floor)
Luca Venturini , University of Verona
Alberto Ferrarini , University of Verona
Andrea Minio , University of Verona
Genny Buson , University of Verona
Mario Pezzotti , University of Verona, Verona, Italy
Sara Zenoni , University of Verona
Marianna Fasoli , University of Verona
Massimo Delledonne , University of Verona, Verona, Italy
The great phenotypic diversity between plant varieties is a manifestation of a major variation at the genetic level, which so far has been largely described as differences relative to a specific reference genome and its annotation. The natural diversity cannot be entirely captured by this approach, as many ecotypes feature quite divergent loci and possess genes which are not shared with the rest of the species – a situation described accurately by the term pan-genome. A well-thought RNA-Seq experiment on a variety can dodge these problems altogether by reconstructing the whole transcriptome, leading to a clear view of the single-base differences as well as to the detection of peculiar genes and isoforms

We applied this strategy to Vitis vinifera cv Corvina in order to characterize its genetic diversity by discovery its proprietary set of genes. Initially we sequenced pooled cDNAs isolated from 45 different conditions and organs, leading  to the identification of 45 sequences specific of our variety which are enriched on vacuole transport and cell wall modulation.