W178 CaPrx, a class III peroxidase gene from Coffea arabica specifically induced by root-knot nematode infection

Date: Sunday, January 15, 2012
Time: 3:50 PM
Room: Sunset
Fabio E. Severino , IB - UNESP - Department of Genetics, Botucatu, Brazil
Marcos Brandalise , IB - UNESP - Department of Genetics, Botucatu, Brazil
Carolina S. Costa , IB - UNESP - Department of Genetics, Botucatu, Brazil
Mirian P. Maluf , Embrapa Café, Brasília - DF, Brazil
Ivan G. Maia , IB - UNESP - Department of Genetics, Botucatu , Brazil
Class III peroxidases (Prx) are enzymes involved in a multitude of physiological and stress-related processes in plants. Here, we report on the characterization of a peroxidase coding gene from Coffea arabica (CaPrx) that is expressed in early stages of root-knot nematode (RKN) infection. CaPrx showed enhanced expression in coffee roots inoculated with RKN (at 12 h post-inoculation), but no difference in expression was observed between susceptible and resistant plants. In contrast, CaPRx was not responsive to mechanical injury indicating that the observed up-regulation is not related to a wound response. Assays using transgenic tobacco plants harboring a promoter-Beta-glucuronidase (GUS) fusion revealed that the CaPRx promoter was exclusively active in the galls induced by RKN. In cross sections of galls, GUS staining was predominantly localized in giant cells. Up-regulation of GUS expression in roots of transgenic plants following RKN inoculation was observed within 16 h. Altogether, these results point to a putative role of this peroxidase in the general coffee response to RKN infection.