W189 The sunflower genome and its evolution

Date: Tuesday, January 17, 2012
Time: 2:20 PM
Room: Pacific Salon 4-5 (2nd Floor)
Nolan Kane , University of British Columbia, Vancouver, BC, Canada
Christopher J. Grassa , University of British Columbia - Department of Botany, Vancouver, BC, Canada
Navdeep Gill , University Of British Columbia, Vancouver, BC, Canada
John Bowers , Department of Plant Biology, University of Georgia
Helene Berges , INRA-CNRGV, Castanet Tolosan, France
Jérôme Gouzy , Laboratoire Interactions Plantes Micro-organismes, INRA/CNRS, Castanet-Tolosan , France
Matthew King , Pioneer Hi-Bred, Johnston, IA
Eleni Bachlava , Monsanto Company
Nicolas Langlade , INRA LIPM, Castanet-Tolosan, France
John M Burke , University of Georgia, Athens, GA
Patrick Vincourt , INRA LIPM, Castanet-Tolosan
Steven J. Knapp , The Monsanto Company
Loren Rieseberg , University of British Columbia, Vancouver, BC, Canada
Here we report a draft sequence of the 3.6 Gb genome of cultivated sunflower (Helianthus annuus), the most economically important crop in the Compositae family. The genome was put together using a combination of 24x 454 whole-genome shotgun (WGS) sequencing and 80x Illumina WGS, along with ultra-high density genetic and physical maps that serve as scaffolds for the linear assembly WGS sequences. Of particular importance, our sequence-based physical map covers 97% of the sunflower genome, with unique sequence tags every 3-6 kb. Assemblies using CABOG, Newbler, ABySS, and SOAPdenovo are compared, and new methods of dealing with highly repetitive sequences are discussed.