W204 “Development and characterization of near isogenic lines (NILs) for agronomic traits in chickpea”

Date: Saturday, January 14, 2012
Time: 2:30 PM
Room: Pacific Salon 6-7 (2nd Floor)
Teresa Millan , University of Cordoba, Cordoba, Spain
Latifeh Ali , University of Cordoba, Cordoba, Spain
Patricia Castro , University of Cordoba, Cordoba, Spain
Eva Madrid , University of Cordoba, Cordoba, Spain
Juan Gil , University of Cordoba, Cordoba, Spain
Josefa Rubio , Area de Mejora y Biotecnologia, IFAPA, Cordoba, Spain
Near Isogenic Lines (NILs) for agronomic traits like disease reaction to Fusarium oxysporum f. sp. ciceris (Foc), flowering time, seed color, growth habit, double/single pod and nodulation/non-nodulation capacity were obtained in chickpea. NILs were developed by searching in segregating advanced recombinant inbred line (RIL) populations derived from both intra and interspecific crosses. Four pairs of these NILs were characterized for disease reaction to Foc races 1A, 2, 3, 4 and 5 for both resistance/susceptibility and slow wilting/susceptibility reactions. The remaining pairs of NILs for late/early flowering, erect/prostrate growth habit, colored/no-colored seeds and nodulation/no-nodulation capacity were evaluated under field conditions. All plant materials were genotyped using molecular markers in order to confirm expected genetic background in different pairs. Moreover, a crossing program between validated pairs of NILs was initiated to obtain new segregating populations for fine mapping. These NILs have great potential for studying the genetics and mechanisms of wilt resistance, identification of new markers linked to traits of interest, fine mapping or expression studies.