Date: Saturday, January 14, 2012
Time: 2:10 PM
Time: 2:10 PM
Room: Pacific Salon 6-7 (2nd Floor)
Chickpea (Cicer arietinum L.) is the world’s third most important food legume cultivated in arid- and semi-arid tropics. It is a self-pollinated, diploid (2n=2x=16), annual plant with a moderate genome size of ~740 Mb. Two new Bacterial Artificial Chromosome (BAC) libraries (one digested with HindIII and the other one with EcoRI) for the reference chickpea genotype (ICC 4958) have been constructed. High information contig fingerprinting (HICF) of 69,984 BAC clones (35,040 clones from HindIII and 34,944 clones from EcoRI libraries) gave high-quality fingerprinting data for 67,164 clones. Fingerprinting of an additional 1144 BAC clones, 773 of which correspond to NB-LRR disease resistance gene clusters (UC-Davis) and 371 of which have been genetically mapped via BAC-end derived SSRs (BES-SSR) (Thudi et al. 2011, PLoS One), was also completed. 46,112 clones were assembled into 1,174 contigs, with 3,256 singleton clones. The average contig size is 0.49 Mb, with a maximum contig size of 4.1 Mb. Taken together, the existing physical map spans an estimated 574 Mb. Genetic map positions for 245 BES-SSR markers permits an intial integration of BAC contigs with the chickpea genetic map. Efforts are underway to define the minimum tiling path (MTP) based on the available physical mapping data, which will facilitate either BAC-end or pooled BAC- sequencing of MTP clones. The resulting integrated genetic and physical map is expected to enhance genetics and genomics research and breeding applications in chickpea.