P0448 Introgression and Gene Expression Differences in Common Bean (Phaseolus vulgaris L) Responsible for White Mold

Sujan Mamidi , Department of Plant Sciences, North Dakota State University, Fargo, ND
Erin Felicetti , USDA-ARS, Prosser, WA
Samira Moghaddam , Genomics and Bioinformatics Program, North Dakota State University, Fargo, ND
Rian Lee , Department of Plant Sciences, North Dakota State University, Fargo, ND
Phillip Miklas , USDA-ARS, Prosser, WA
Phillip McClean , Department of Plant Sciences, North Dakota State University, Fargo, ND
White mold is an important foliar disease that effects production of common bean (Phaseolus vulgaris L.) in the United States. It is caused by the necrotrophic fungus Sclerotinia sclerotiorum de Bary. Resistant sources of the disease provide a better control than cultivation practices and fungicide application. Tolerance is provided by 20 QTL located on nine of the eleven common bean chromosomes. We used RNA-Seq data to map the introgressed regions. We used a pooled sample of isogenic lines, with and without the WM8.3 QTL which was identified earlier.  We identified two main regions of introgression, on chromosomes 7 and 8. We identified ~500 genes differentially expressed in resistant and susceptible at 24 hrs and 48 hrs with just a few genes in common at the two time points. The importance of these genes in white mold tolerance will be discussed.