Date: Saturday, January 14, 2012
Time: 1:50 PM
Time: 1:50 PM
Room: Pacific Salon 6-7 (2nd Floor)
Lentil (Lens culinaris) has been late to enter the world of high throughput molecular analysis due to a general lack of genomic resources. Using a 454 sequencing-based approach, SNPs have been identified in genes across the lentil genome. Several hundred have been turned into single SNP KASP assays (KBioscience) and a 1536-SNP Illumina Golden Gate assay has been developed. A collection of over 300 diverse lines has been genotyped to assess genetic diversity and structure in lentil germplasm. A gene-based map of the lentil genome has been developed that has been used to identify regions of conserved synteny with Medicago truncatula. Phenotypic evaluation of both bi-parental and association mapping populations is underway and is leading to the identification of both markers and genes associated with traits of interest to the lentil breeding program.