BAC (bacterial artificial chromosome) and BIBAC (binary BAC) resources are important tools for positional cloning, whole or targeted genome sequencing, comparative mapping and large DNA fragment transformation. To support genomics studies, we have set up a public BAC/BIBAC resource platform in China (http://GResource.hzau.edu.cn). A pair of new BAC and BIBAC vectors were constructed that can facilitate BAC/BIBAC library construction, insert sizing and intact insert exchanging between the two vectors. BAC physical maps for Chinese elite rice varieties Zhonghua 11, 93-11, Zhenshan 97 and Minghui 63 were constructed in collaboration with Arizona Genomics Institute. These BAC resources have been used for different purposes. Recently, a set of ZMAP BAC/BIBAC libraries were constructed for maize (Zea) varieties and close relatives including the BAC libraries for maize Mo17, Zheng 58, Chang 72, Parviglumis and Tripsacum and BIBAC libraries for maize B73 and a sorghum variety. We are massively transforming the maize B73 BIBACs into rice plants. Currently, more than 1,000 positive rice plants transformed with random B73 BIBAC clones have been obtained. We have demonstrated that a 164-kb maize B73 genomic DNA fragment with 88.1% repeat sequences and containing 5 filtered gene models, was completely integrated into rice genome. Some transgenic rice plants with changed phenotypes have been obtained and are being further analyzed. We are mapping the B73 BIBAC library to the B73 genome sequence and are trying to construct transgenic rice lines with tiling maize B73 BIBACs.