P0880 The Chemoterapeutic Cisplatin Changes The Expression Of Genes Related To Antioxidant Defense And Oxidative Stress In Mice

Juliana Mara Serpeloni , Universidade de São Paulo (USP), Ribeirão Preto, Brazil
Cisplatin (cDDP) is one of the most potent chemotherapy agents used to treat a variety of tumors because of its ability to induce DNA damage and the formation of reactive species (RS). The main goal of this study was to evaluate the effects of cDDP is linked to changes in gene expression. For this purpose, 84 genes were analyzed in livers of mouse treated with cDDP using quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) array technique. The animals were divided into two groups (N=8). Mineral oil was administrated by gavage to all animals for 13 consecutive days.  In the 14th day, mice received saline (control group) or cDDP injection (cDDP group) and 24 hours after that the animals were euthanized to remove liver samples. RT2 ProfilerTM PCR array was performed for mouse oxidative stress and antioxidant defense pathways and were considered as differentially expressed positive or negative values of expression greater than or equal to 2.0. cDDP increased the expression of genes encoding 9 antioxidants proteins and 3 involved in RS metabolism and oxygen transporters (mainly Serpinb1b and Ccs). cDDP also decreased the expression of 6 antioxidants genes and 10 involved in RS metabolism and oxygen transporters (mainly Idh1 and Prnp). Overall, our results suggest that exposure to cDDP contributed to disequilibrium in the redox status of cells not only depleting antioxidant defenses but also decreasing the expression of important antioxidant genes and also affecting the metabolism of RS metabolism.