Spiders possess distinctive evolutionary adaptations, such as venom and silk production, providing an ample system for evolutionary and developmental studies of anatomical adaptation. Here we compare transcripts from 2 suborders of the Araneae order: Araneomorphae and Mygalomorphae. We've constructed EST libraries from the spinning gland of the Araneomorphae Gasteracantha cancriformis (52,399 454 GS-FLX Titanium reads) and the Mygalomorphae Actinopus spp. (454 GS-FLX Titanium 34,496 reads) and analyzed those together with the 22,812 Sanger ESTs from Parasteatoda tepidariorum, 3,201 Sanger ESTs from 2 Loxosceles species (Araneomorphae spiders), 6,790 Sanger ESTs from Acanthoscurria gomesiana and 2,697 Sanger ESTs from Aphonopelma sp. (Mygalomorphae spiders) currently available at GenBank.