W617 Searching for SNP, Indel, and TE Transposition in a T-DNA Tagged Rice Mutant Line

Date: Sunday, January 15, 2012
Time: 11:40 AM
Room: Golden West
Fu-Jin Wei , Institute of Plant and Microbial Biology, Academia Sinica, , Taipei, Taiwan
Hung-Ying Lin , Institute of Plant and Microbial Biology, Academia Sinica, , Taipei, Taiwan
Lin-tzu Huang , Institute of Plant and Microbial Biology, Academia Sinica, , Taipei, Taiwan
Cristian Chaparro , University of Perpignan, Perpignan, France
Olivier Panaud , University of Perpignan, Perpignan, France
Yue-ie Hsing , Institute of Plant and Microbial Biology, Academia Sinica, , Taipei, Taiwan
With the completion of the rice genome sequencing project, the next major challenge is the large-scale determination of gene functions. The detail phenomics analysis is the key for functional genomics. By T-DNA insertional mutagenesis approach, we have generated a rice mutant population containing promoter trap and gene activation/knockout lines using a japonica rice cultivar Tainung 67 (TN67), with the website at http://trim.sinica.edu.tw. About 60% of them consist of known integration sites and 80% with phenomics records. About 18% of the scored mutant lines consist of mutant phenotypes, 90% of them have one type of mutant type and the rest have two or more types. Thus, mutations must include those induced by the integrated T-DNA, the movement of Tos17, as well as other sequence changes of unknown origin. We will present the recent TRIM utilization and possible mechanisms for somaclonal variation, including SNP, indel, and TE transposition in one of these lines.