Restriction-Site Associated DNA Sequencing (RAD-Seq)

Next-generation sequencing of restriction-site associated DNA (RAD-Seq) is a genotyping method widely applicable to a variety of biological problems. As sequencing costs have dropped, RAD-Seq has moved from being primarily a SNP discovery tool to being a method for high-throughput genotyping of populations. We will present case studies of RAD-Seq projects ranging from SNP discovery in complex genomes lacking a reference to population studies, with a discussion of project design and caveats to interpreting RAD-Seq data. We will also discuss variations to the RAD method and future improvements to the method.