P0093 A Genome-Wide Survey of Switchgrass Genome Structure and Organization

Manoj K. Sharma , University of California Davis, Davis, CA
Rita Sharma , University of California Davis, Davis, CA
Peijian Cao , Zhengzhou Tobacco Research Institute, Zhengzhou, China
Jerry Jenkins , HudsonAlpha Institute of Biotechnology, Huntsville, AL
Laura Bartley , University of Oklahoma, Norman, OK
Jane Grimwood , HudsonAlpha Institute of Biotechnology, Huntsville, AL
Jeremy Schmutz , HudsonAlpha Institute of Biotechnology, Huntsville, AL
Pamela Ronald , Joint BioEnergy Institute, Emeryville, CA
The perennial grass, switchgrass (Panicum virgatum L.), is a promising bioenergy crop and the target of whole genome sequencing. We constructed two bacterial artificial chromosome libraries from the AP13 clone of switchgrass to gain insight into the genome structure and organization, initiate functional and comparative genomic studies, and assist with genome assembly. Together representing 16 haploid genome equivalents of switchgrass, each library comprises 101,376 clones with an average insert size of 144 (Hind III-generated) and 110 kb (BstY I-generated). A total of 330,297 high quality BAC-end sequences (BES) were generated, accounting for 263.2 Mbp (16.4%) of the switchgrass genome. Analysis of the BES identified 279,099 known repetitive elements, >50,000 SSRs and 2,528 novel repeat elements, named switchgrass repetitive elements (SREs). A total of 48,000 clones from each library were organized into pools and superpools (~7X coverage) for efficient qPCR-based screening. 176 BACs carrying cell wall and defense response-related genes were selected and sequenced to full-length providing complete genomic sequences of rice orthologs of 259 kinases, 118 gylcosyltransferases, 84 glycoside hydrolases and 13 ethylene response factors (ERFs). Comparative mapping of 100 full-length BAC sequences and 330K BES revealed high levels of synteny with the grass genomes sorghum, rice, maize and Brachypodium. Our data indicate that the overall sequence composition of the switchgrass genome is most similar to that of rice and that the sorghum genome has retained larger microsyntenous regions with switchgrasse. The resources generated in this effort will be useful for a broad range of applications.