P0019 The Oryza BAC resource for exploring genome evolution

Jayson Talag , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Xiang Song , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Siva S. Ammiraju Jetty , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Dave Kudrna , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Wolfgang Golser , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Yeisoo Yu , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Kristi Collura de Baynast , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Marina Wissotski , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Jose Luis Goicoechea , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Jianwei Zhang , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Rod A. Wing , Arizona Genomics Institute, University of Arizona, Tucson, AZ
Rice was the first crop to have a high-quality reference genome sequence and is now at the forefront of intense functional and evolutionary research for two reasons - its central role in world food security, and its status as a model system for grasses.  A thorough characterization of the rice genome cannot be accomplished without a deep understanding of its evolutionary history. The genus Oryza contains two cultivated and 22 wild rice species that represent 10 distinct genome types embedded within a robust phylogeny spanning ~15 million years. The genus contains an untapped reservoir of agriculturally important traits and a historical record of genomic changes, especially those related to domestication, polyploidy, speciation and adaption. 

Here we report two new publicly available high quality Oryza BAC resources: O. glumaepatula (AA), and O. meridionalis (AA).  Additionally, we report the first availability of BAC clone resources for Leersia perrieri  (genome size of 323Mb), the closest evolutionary relative to the Oryza genus separated by ~4 million years.   BAC clone end-sequences, BAC clone fingerprints and phase I physical maps have been generated to establish syntenic alignments of these new resources to rice.  All resources are available from the Arizona Genomic Institute Resource Center (http://www.genome.arizona.edu/orders/ ). This work is funded by the NSF Plant Genome Program, #1026200.