P0090 Non gridded and Low coverage BAC libraries: New approaches to explore plants genetic diversity

Genséric Beydon , INRA-CNRGV, Castanet Tolosan, France
Sonia Vautrin , INRA-CNRGV, Castanet Tolosan, France
Arnaud Bellec , INRA-CNRGV, Castanet Tolosan, France
Clémentine Vitte , CNRS - UMR de Génétique Végétale, Gif sur Yvette, France
Rohit Mago , CSIRO Plant Industry, Canberra, Australia
Wolfgang Spielmeyer , CSIRO Plant Industry, Canberra, ACT, Australia
Helene Berges , INRA-CNRGV, Castanet Tolosan, France
The French Plant Genomic Resource Centre (CNRGV) is a genomic platform dedicated to assist the scientific community for plant genomics projects. The CNRGV aims to construct and manage genomic libraries and to provide efficient tools and services to develop genomic projects. The CNRGV holds more than 8 millions unique samples among more than 70 plant BAC and cDNA libraries. BAC libraries are an invaluable tool for genome analysis, physical mapping, map-based cloning and sequencing projects. BAC clones permit to individualize DNA large fragments thus allow to unravel genome's complexity in polyploid and repeated contexts. The emergence and spread of 2nd generation sequencing technologies combined with these unique abilities has reinforced the interest of BAC libraries in plant for de novo sequencing strategies. Numerous BAC libraries currently exist for most of cultivated or model plants. These resources combined with genetic mapping programs have permit to link genomic regions of interest with various phenotypic characters. However understanding of specific alleles and improvement of agronomical traits require to investigate these specific genomic regions of interest within correlated genotypes. To meet this need we have developed new BAC library construction strategies: (i) non gridded BAC library and (ii) low-coverage BAC library. These two strategies are pragmatic approaches which aim to avoid BAC clones massive picking steps and to reduce screening effort. Thus we can access rapidly to sequence diversity among various ecotypes. These strategies has proven their efficiency through various projects completed at CNRGV in collaboration with scientists. Two projects will be presented :

- Genome structure comparison of various maize genotypes at targeted loci using low coverage strategy

- Construction of non gridded BAC libraries of wheat specific genotypes to isolate genes of interest based on data from well studied genotype Chinese spring