P0711 A high-density RH map genes and its direct link to the recently completed goat assembly

Xiaoyong Du , Huazhong Agricultural University, Wuhan, China
Bertrand Servin , Toulouse, INRA, Laboratoire de Génétique Cellulaire and Biométrie et Intelligence Artificielle, Castanet-Tolosan, France
T Faraut , Toulouse, INRA Laboratoire de Génétique Cellulaire and Biométrie et Intelligence Artificielle, Castanet-Tolosan, France
James Womack , Texas A&M University, College Station, TX
Wen Wang , Kunming Institute of Zoology, Kunming, Yunnan, China
Massoud Malek , International Atomic Energy Agency, Vienna, Austria
Shu-Hong Zhao , Huazhong Agricultural University, Wuhan, Hubei Provinc, China
Goat products are important diet protein supply and are the main income source for many farmers in rural areas in the world. Three-fourths of the goats are raised in developing countries. They can live in harsh environments, making otherwise unusable land productive. There are more than 40 local goat breeds in China. However, the performances of the local breeds are low due to inbreeding, poor management, and little effort made to the goat genetic improvement. Moreover, lack of its genomic maps greatly hindered the study of goat breeding and genetics. In this study, we generated a 5000-rad goat radiation hybrid panel. Of the 74 goat, sheep, and cattle derived markers were initially selected for genotyping against the panel, 44 were mapped in three linkage groups in chromosome 1. A robust RH map of whole goat genome using improved methods was constructed. In total, 30,988 SNP markers are included in the robust map and cover all 30 caprine autosomes and the X chromosome at an average spacing of approximately 85 Kb. This high-density RH Map has been used to verify accuracies of the de novo goat assembly. Comparing to other ruminant genome, the marker orders in our RH map have a consistence of 96.1% with sheep genome, whereas of 90.9% with cattle genome.