Time: 2:15 PM
Along with the accumulation of genome information, the demand for an efficient experimental tool for gene function analysis has been increasing. Reverse genetics is one of the effective approaches. In order to establish the basis for reverse genetic approach, we are currently preparing large scale insertion tag lines applying endogenous retrotransposon of L. japonicus named LORE1. Since LORE1 transposition occurs in the germline, seeds harvested from a single activated line have different insertion sites in their genomes, and thus can be considered as independent tag lines. The large-scale detection of LORE1 insertion sites using second generation sequencers revealed a preference for genic regions, especially in exons of protein coding genes. With these features suitable for gene tagging line construction combined with established high-throughput FST analysis system, LORE1 insertion lines pave the way to development of a comprehensive reverse genetic resource.