P0342 The barley physical map impact of different BAC libraries made of partially digested or randomly sheared genomic DNA

Ruvini T. Ariyadasa , Leibniz- Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
The development of a genome-wide physical map for barley (Hordeum vulgare cv. Morex , genome size=5Gbp) was initiated and coordinated by the International Barley genome sequencing consortium (IBSC, Schulte et al., 2009). Six BAC libraries were developed from HindIII (3X), EcoRI, MboI and random sheared. From these libraries ~650,000 BAC clones were fingerprinted via high information content fingerprinting (HICF). A total of 570,000 (13X genome coverage) high quality fingerprints were selected and used for de novo contig assembly (FPC V9.2). The final contig assembly is composed of almost 10,000 BAC contigs.  By taking advantage of a diverse set of anchoring information, 80% of the physical length of the contig assembly was anchored to the genetic map.  The different BAC libraries were examined to determine which type of library gave the greatest contribution to the physical map and the level of bias in whole genome representation. An overview of the status of the barley physical map will be presented.