Integration sites of transformation events in the sugar beet genome

We have compared the integration sites of Agrobacterium-induced transformants in the genome of sugar beet (B. vulgaris), to examine the relationship between sequence environment and the effectiveness of transgene expression.  Sets of independent events, taken from two series of transformants, were characterised for their genomic insertion sites. Genome walking was performed from border sequences into genomic flanking regions, and the sequences obtained used to identify their homologs in the SESVanderHave whole-genome assembly.

Data will be presented on their proximity to annotated genes and other features in the surrounding sequences, and the relationship between transgene and flanking region expression levels.