P0867 Transcriptome Analysis and Glycome Profiling of three Eucalyptus species

Marcela M. Salazar , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Sivakumar Pattathil , Complex Carbohydrate Research Center - University of Georgia, Athens, GA
Eduardo L. O. Camargo , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Adriana Grandis , Laboratory of Plant Physiological Ecology - University of Sao Paulo, Sao Paulo, Brazil
Danieli C. Gonçalves , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Leandro Costa Nascimento , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Wesley L. Marques , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Jorge lepikson-Neto , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Marcelo F. Carazzole , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
Marcos S. Buckeridge , Laboratory of Plant Physiological Ecology - University of Sao Paulo, Sao Paulo, Brazil
Michael G. Hahn , Complex Carbohydrate Research Center - University of Georgia, Athens, GA
Gonçalo Amarante Guimarães Pereira , Genomics and Expression Laboratory - State University of Campinas - UNICAMP, Campinas, Brazil
The genus Eucalyptus is composed of more than 600 species with high phenotypic and adaptation variability, allowing for different utilization of its wood. Three species are among the most planted: E. globulus, E. grandis and E. urophylla, They contain remarkable differences in lignin content, syringyl/guayacyl (S/G) ratio, cellulose productivity and adaptability to tropical conditions. Despite the importance of these species, information about gene expression and cell wall composition are still incomplete, impairing our ability to compare and comprehend the differences between these three species.  The goal of the present work was to investigate the glycan diversity and content of these three Eucalyptus species using a new high-throughput approach named Glycome Profiling, which uses a large and diverse collection of 156 plant glycan-directed monoclonal antibodies. The results were combined with molecular data produced by xylem RNAseq transcriptome analyses. Our results suggest that significant differences in cell wall composition and structure occur among the three species.  The high glucose yield obtained from E. globulus is probably a result of different connections among cell wall molecules that allows facile degradation of cellulose.  The observed differences can be, partly, explained by differential gene expression of cell wall related genes.  These are pioneering data to provide an overview of glycan content and distribution in Eucalyptus and also a transcriptome comparison for these three species.  Understanding the process of wood formation, composition and structure of cell wall components is critical for planning genetic improvement programs to develop less recalcitrant and higher yielding bioenergy feedstocks.