NEBNext fast and simple library construction for the Ion Torrent PGM

As the quantity of data generated per next generation sequencing (NGS) run increases and the time required per run decreases, the ability to quickly produce large numbers of libraries inexpensively is becoming increasingly important.   For example, sequencing runs on the ION Torrent PGM take approximately two hours, substantially less time than it takes to construct a single library.  Moreover, the increased throughput per run enables the simultaneous sequencing of multiple samples. These advances in massively parallel DNA sequencing have resulted in library construction becoming a bottle neck to sequencing. To address this problem, we have developed a fast and simple library construction pipeline for the ION Torrent PGM.  Using newly developed NEBNext reagents, libraries are constructed in approximately two hours, with a streamlined method that combines multiple steps in a single tube. As a result, the time required to construct libraries is greatly reduced with minimal hands on time. The new reagent set is compatible with single sample preparation or multiplexing and can be used with low input DNA samples.