The introgression of new genetic variation into B. napus canola breeding material has the potential to significantly increase the overall agronomic performance of the crop and offers the prospect of developing canola varieties with improved production and quality traits. Genome-wide introgression facilitates the determination of the effect of individual chromosome segments in a common genetic background. Our objective is to introgress genetic variation into an elite canola variety from B. napus resynthesized from progenitor diploid species and develop genome-wide introgression lines. Well characterized re-synthesized B. napus cv. PSA12 was used as a source of genetic variation. A DH population generated from the F1 plants of elite cultivar x PSA12 was genotyped with SSR and SNP markers to select a subset of lines representing the total donor genome of PSA12. Results of this selection and also the alignment of genetic map will be presented.