All-in-one kit solution for simple, rapid, consistent algae engineering

Date: Monday, January 16, 2012
Time: 2:30 PM
Room: Golden West
Todd Peterson , Life Technologies, Carlsbad, CA
GeneArt® Algae Engineering Kits are the first available genetic modification and expression systems for photosynthetic microalgae Chlamydomonas reinhardtii and Synechococcus elongatus.  The strains are model organisms for the study of photosynthesis, circadian rhythms and nutrient-regulated gene activity, and are being investigated for bioproduction of biofuels, nutraceuticals and specialty chemicals.  The Kits combine optimized and fully characterized vectors, guaranteed pure and viable cells, specialty media for each strain and simple protocols to create the first standardized, complete system for algae research and metabolic engineering. The algal strains contained in the kits are the first that can be shipped and stored frozen at -80 degrees Celsius, which simplifies their standardization and use.  The GeneArt® vectors facilitate rapid, directional cloning of synthetic genes or PCR products and serve dual purpose for both engineering and expression.  Gibco® media is optimized for each strain and provided at 1X concentration, eliminating laborious and time-consuming media preparation. The kits will allow researchers to create transformed algal cells in fewer days, to standardize experiments with genetically consistent frozen cell stocks and to save time that might otherwise be spent on strain optimization, media preparation and other labor-intensive steps.