De novo Sequencing of Complex Plant Genomes

De novo sequencing of complex genomes has been increasingly utilized for research on non-model organisms, unculturable species (metagenomics), or comparative transcriptome analysis. Roche’s GS FLX+ System features the latest innovations in 454 Sequencing reagents and software to support accurate de novo sequencing of complex genomes. With read lengths of up to 1,000 base pairs, the GS FLX+ System enables a broad range of applications, including DNA shotgun sequencing, RNA transcript analysis, and targeted resequencing to generate optimal assemblies for accurate assignment of repeat regions.  454 extra-long reads are an ideal tool to bridge through inverted repeats, extensive gene duplications, and multiple inversions found in many plants – enabling variant comparison between strains or within genomes. The GS FLX+ System provides a powerful combination of features enabling detailed analysis of assembled sequencing data. This presentation will demonstrate the utility of 454 Sanger-like read lengths for accurate assemblies, leading to a better understanding of plant evolution for food or bioenergy research as well as environmental studies.

For life science research only. Not for use in diagnostic procedures.

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