P0040
Amplicon Based 16S Ribosomal RNA Sequencing and Genus Identification - Mixed Species Metagenomics

Date: Monday, January 12, 2015
Room:
Masoud Toloue , Bioo Scientific, Austin, TX
Jan Risinger , Bioo Scientific, Austin, TX
Lindsey Renken , Bioo Scientific, Austin, TX
Josh Hill , AgriLife Genomics and Bioinformatics Service, Texas A&M University, College Station, TX
Noushin Ghaffari , AgriLife Genomics and Bioinformatics Services, College Station, TX
Richard P Metz , AgriLife Genomics and Bioinformatics Services, College Station, TX
Charles D. Johnson , Texas A&M AgriLife, College Station, TX
Next generation sequencing analysis of 16S ribosomal RNA (rRNA) is commonly used to identify bacterial species and perform taxonomic studies. Bacterial 16S rRNA genes contain 9 hyper-variable regions with considerable sequence diversity among different bacterial species and can be used for species id.  Rapid determination of highly complex bacterial populations through targeted amplification can provide an accurate gauge of diversity at taxonomic hierarchies as low as the genus level. A single 16S rRNA hyper-variable domain does not have enough sequence diversity to distinguish genera. With increased read lengths of MiSeq chemistry, Bioo Scientific has expanded the common analysis of the fourth hyper-variable domain (V4) of prokaryotic 16S rRNA to V1, V2 and V3 regions simultaneously. Optimized preparation through a streamlined standardized procedure allows for high-quality, reproducible libraries. This optimization can be applied to different windows of 16S rRNA as well as other relevant prokaryotic taxonomic markers.
Handouts
  • Bioo Scientific - Amplicon Based 16S Ribosomal RNA Sequencing and Genus Identification PAGXXIII.pdf (1.2 MB)