W860Old School/New School Genome Sequencing: One Step Backward - a Quantum Leap Forward
Time: 4:20 PM
To date, the highest quality genomes available (e.g. human, Arabidopsis, and rice) have relied on BAC-based physical maps, shotgun sequencing of individual BACs, finishing and assembly. Our lab has been experimenting with a modification of this Old School approach by first: a) generating whole genome profiling sequence-based physical maps of BACs; 2) sequencing pools of BACs (i.e. 24, 32, or 48) with PacBio long read chemistry; and 3) assembling all reads into reference quality genomes.
Data will be presented for a rice genome recently sequenced using this approach.