Promega - Addressing the Challenges of Nucleic Acid Extraction, Quantitation and Amplification

Nucleic acid extraction, quantitation and amplification are key processes in the molecular monitoring of plant genotypes and plant health. The diversity of input materials from seeds, embryos, woody mature tissues and the presence of substances that may inhibit nucleic acid amplification create many challenges. Once nucleic acid is isolated, success in DNA/RNA analysis applications often comes down to using the appropriate amount of input nucleic acid, but common nucleic acid quantitation methods provide only minimal information about nucleic acid quality. In this workshop we will review extraction of nucleic acid from a wide range of plant materials using manual to high throughput automated protocols and the relative strengths of absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation of purified nucleic acids to allow you to select the appropriate methods for your sample type and downstream application.
Date: Tuesday, January 12, 2016
Time: 1:30 PM-3:40 PM
Room: Sunset - Meeting House
Mary Jo Martinson
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