High-Throughput Ultrasonic DNA Shearing for NGS Sample Preparation

As Next-Generation Sequencing (NGS) throughput continues to accelerate, a serious sample preparation bottleneck is emerging, calling for a higher throughput DNA shearing technique.  The solution must not compromise the quality of the sheared fragments and should not add to the existing cost of sample preparation.  Microsonic Systems Inc. (Microsonics) has developed a unique, new ultrasonic technology “Bulk Lateral Ultrasonic (BLU)™ energy” and created a core building block the Microprocessor for Life Sciences™ to commercialize this exciting new technology. Together, these form the basis for a multi-channel DNA shearing device that will shear multiple DNA samples simultaneously.

This poster will explain how Microsonics’ ST104, its first high-throughput shearing instrument, applies to NGS sample preparation, including:  1. DNA shearing fragment size range, 2. Sample concentration independence, 3. DNA shearing reproducibility, and 4. Economic benefits.