Date: Saturday, January 14, 2012
Time: 1:30 PM
Time: 1:30 PM
Room: San Diego
In 2006 the International Barley genome Sequencing Consortium (IBSC) teamed up to construct a barley physical map, with the end goal to use this resource to facilitate development of a high quality reference sequence for this important cereal crop. The overall objective of the IBSC is to characterize the barley gene space, with the near-term need being the identification of the remainder of ~50,000 genes, including the 5’ and 3’ regulatory regions, and the longer-term goal an ordered physical map linked to the genetic map to accelerate crop improvement. Since 2006, a BAC based physical map has been advanced to 13-fold haploid genome coverage by classical High Information Content Fingerprinting. Less than 9,500 BAC contigs were obtained that span over 95% of the physical length of the barley genome. 1,500 contigs represent over 50% of the barley genome. 3,500 BAC clones (440 Mbp) were shotgun sequenced by Next Generation Sequencing (NGS) technology providing access to about 8% of the genome sequence. NGS BAC sequencing delivered important information for anchoring of the physical map but also helped further the strategy towards sequencing of the entire genome. A complex set of information of sequenced BAC ends, gene-based markers / BAC clone relationships, a de novo whole genome shotgun sequence assembly, and large sets of genetically mapped sequence tags will be integrated with the BAC contig map eventually allowing to anchor ~80% of the physical map to the genetic map of barley.