Sequences of 14,600 Gene-Bearing Minimal Tiling Path BACs of Morex Barley

In a project entitled “Advancing the Barley Genome”, supported by the USDA-AFRI-NIFA program, we applied combinatorial pooling and Illumina sequencing to ~14,600 BACs that constitute a minimal tiling path (MTP) from 83,831 gene-bearing BACs of Morex barley. The BAC library was developed by Yu et al. (2000, TAG 101:1093-99) and these BACs account for roughly 70% of all expressed genes but only about 30% of the entire barley genome. Identification of gene-bearing BACs, fingerprinting and initial assignment of BACs to the genetic map were the result of prior projects supported by NSF and USDA and contributions from many additional individuals who will be cited in the workshop presentation. The newly generated sequences, in conjunction with knowledge of barley and other grass genomes, have enabled the anchoring of all but a small percentage of these gene-bearing BACs to the genetic linkage map. The sequences are available as a single concatentated FASTA file for each BAC, with headers providing map information. HarvEST:Barley (http://harvest.ucr.edu) and utilities available from www.harvest-web.org and www.harvest-blast.org provide interfaces to navigate the resulting gene-rich portion of the barley genome and facilitate data access. Updates on data portals and integration of the new results into International Barley Sequencing Consortium (IBSC) resources can be found at the IBSC website (www.barley-genome.org).