The Rotylenchulus reniformis Genome: Sequencing, Assembly, and Annotation

Sanders, William S.

William S. Sanders , Institute for Genomics, Biocomputing, and Biotechnology - Mississippi State University, MS State, MS

Kurt Showmaker , Institute for Genomics, Biocomputing, and Biotechnology - Mississippi State University, MS State, MS

Ben Bartlett , Institute for Genomics, Biocomputing, and Biotechnology - Mississippi State University, MS State, MS

Hui Wang , Institute for Genomics, Biocomputing, and Biotechnology - Mississippi State University, MS State, MS

Satish Ganji , Department of Biochemistry & Molecular Biology - Mississippi State University, MS State, MS

Martin Wubben , USDA, ARS, Mississippi State, MS

Fiona McCarthy , Mississippi State University, MS State, MS

Zenaida Magbanua , Department of Plant and Soil Sciences - Mississippi State University, MS State, MS

Daniel G. Peterson , Institute for Genomics, Biocomputing & Biotechnology - Mississippi State University, Mississippi State, MS

Rotylenchulus reniformis (the reniform nematode) is a plant parasitic nematode with a primarily tropical and subtropical geographic distribution. The reniform nematode is an emerging problem in US cotton production, causing an estimated annual $130,000,000 USD crop loss. Adult females establish feeding sites in the plant root, where the head portion of the nematode is positioned inside the plant root and the external tail portion begins to swell, developing a kidney-like shape. Understanding of the genome structure of this organism can help provide insight into the mechanisms of its parasitism and aid in the development of reniform resistant cotton lines. Genomic reniform DNA was isolated and sequences were generated using two platforms, combining both Illumina Sequencing-by-Synthesis and Roche 454 Pyrosequencing technologies. Sequences were assembled using the ABySS assembly algorithm. Here we present the current status of the Rotylenchulus reniformis genome sequencing project and discuss the present state of our draft assembly and the associated annotations.

P0066 The Rotylenchulus reniformis Genome: Sequencing, Assembly, and Annotation