Characterization of vacuolar Ca2+ transporter genes in Brassica oleracea

Calcium is an essential plant macronutrient with unique structural and signaling roles. Tip-burn is generally considered to be a calcium-related disorder, caused by localized Ca²⁺ deficiency of leaf margins. We obtained two types of cabbage lines, tip-burn susceptible and resistant. The Ca²⁺ concentration was measured for both of lines by using Fluo-4/AM dye, and ion chromatography. The concentration of Ca²⁺, Mg²⁺, Na⁺ and K⁺ was higher in the bottom of leaves than upper of leaves in both lines. Three cations (Ca²⁺, Mg^{2 +}and K⁺) measured are present >2 times in the tip-burn resistant line. Sodium ion was detected more from susceptible line. We also evaluated the expression patterns of the major vacuolar Ca²⁺ transporters, CAX and ACA in leaves of the lines. The expression pattern of several Ca²⁺ transporter genes was different between tip-burn susceptible and resistant lines. These data imply that different transcript level of vacuolar Ca²⁺ transporters in Brassica oleracea leaf may also indicate that ability of calcium ion transport or degree of storage are different. We will present transcriptome data of tip-burn susceptible and resistance lines on this poster. [This work was financially supported by grants from Cabbage Genomics assisted breeding supporting Center (CGsC) research programs funded by Ministry for Food, Agriculture, Forestry and Fisheries of the Korean Government]