Overview of the Genotyping-By-Sequencing Technology and Its Applications In Diverse Organisms

Genotyping by sequencing (GBS) is a simple highly-multiplexed system for constructing reduced representation libraries for the Illumina next-generation sequencing platform developed in Ed Buckler lab of USDA. This presentation will  give the overview of this technology, current progress, and its applications in constructing genetic maps, trait mapping and genome contig scaffolding in multiple different species. Prospect will be discussed of applying GBS technology, integrated with whole genome sequencing assembly, to study the genetic diversity in coffee, and marker assisted breeding.